Fig. 11. Confirmation of DDX1, hnRNP H2, and hnRNP A2/B1 as targets of 2155-14. (A) Western blot of hnRNP H2 in WM266-4 melanoma cells and melanocytes. (B) Western blot of WM266-4 melanoma and HEK293 cell lysates. Live cells were incubated in presence and absence of 2155-14. (C) Quantification of western blots for (B). (D) Western blot of DDX1, hnRNP H2, and hnRNP A2/B1 in WM266-4 cell lysates after digestion with pronase in the presence and absence of 2155-14. (E) Quantification of western blots for (D). (F) Western blot of DDX1, hnRNP H2, and hnRNP A2/B1 in HEK293 cell lysates after digestion with pronase in the presence and absence of 2155-14. (E) Quantification of western blots for (F). One-way analysis of variance (ANOVA) was used followed by Dunnett post hoc test. The data shown were the mean ± SD, n=3. ***** - p<0.0001, *** - p<0.001, ** - p<0.01, * - p<0.05, rest = no significance.